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Human CCL8 ELISA Kit

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  • Alternative name

    Human HC14 ELISA Kit;Human monocyte chemoattractant protein 2 ELISA Kit;Human monocyte chemotactic protein 2 ELISA Kit;Human MCP-2 ELISA Kit;Human small-inducible cytokine A8 ELISA Kit;Human MCP2 ELISA Kit;Human SCYA10 ELISA Kit;Human SCYA8 ELISA Kit;Human C-C motif chemokine ligand 8 ELISA Kit;Human C-C motif chemokine 8 ELISA Kit;Human chemokine (C-C motif) ligand 8 ELISA Kit;Human small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2) ELISA Kit;
  • Catalog
    E007223
  • species
    Human
  • GeneCCL8
  • SpecificityNatural and recombinant human CCL8
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 1pg/ml
  • Intended UseHuman CCL8 ELISA Kit allows for the in vitro quantitative determination of CCL8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: human CCL8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL8 has been precoated onto 96-well plates. Standards (E.coli, Q24-P99) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL8 amount of sample captured in plate. Background: Chemokine (C-C motif) ligand 8, also known as monocyte chemoattractant protein 2 (MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2 (MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.
  • Human MCP-2(6-76) Protein information
  • Uniprot ID CCL8_HUMAN
  • Uniprot AC P80075; A0AV77; P78388;
  • UniGene Hs.271387;
  • GeneID 6355
  • KEGG hsa:6355;
  • Human MCP-2(6-76) Protein SEQUENCE

  • SEQUENCE 99 AA; 11246 MW; 9D67976BB9422F2A CRC64;

    MKVSAALLCL LLMAATFSPQ GLAQPDSVSI PITCCFNVIN RKIPIQRLES

    YTRITNIQCP KEAVIFKTKR GKEVCADPKE RWVRDSMKHL DQIFQNLKP

  • UCSC uc002hib.4; human.;



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