Mouse Vcl ELISA Kit

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  • Alternative name

    Mouse metavinculin ELISA Kit;Mouse 9430097D22 ELISA Kit;Mouse AA571387 ELISA Kit;Mouse AI462105 ELISA Kit;Mouse AW545629 ELISA Kit;Mouse vinculin ELISA Kit;

  • Catalog
    E072509
  • species
    Mouse
  • GeneVcl
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse VCL. No significant cross-reactivity or interference between mouse VCL and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 7.03 pg/ml
  • Intended UseMouse Vcl ELISA Kit allows for the in vitro quantitative determination of Vcl , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VCL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VCL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VCL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VCL bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Mouse Vinculin Protein information
  • Uniprot ID VINC_MOUSE
  • Uniprot AC Q64727; Q8BP32; Q8BS46; Q922C5; Q922D9;
  • UniGene Mm.279361;
  • GeneID 22330
  • KEGG mmu:22330;
  • Mouse Vinculin Protein SEQUENCE
  • SEQUENCE 1066 AA; 116717 MW; 067F1B16A5285859 CRC64;

    MPVFHTRTIE SILEPVAQQI SHLVIMHEEG EVDGKAIPDL TAPVAAVQAA

    VSNLVRVGKE TVQTTEDQIL KRDMPPAFIK VENACTKLVQ AAQMLQSDPY

    SVPARDYLID GSRGILSGTS DLLLTFDEAE VRKIIRVCKG ILEYLTVAEV

    VETMEDLVTY TKNLGPGMTK MAKMIDERQQ ELTHQEHRVM LVNSMNTVKE

    LLPVLISAMK IFVTTKNSKN QGIEEALKNR NFTVEKMSAE INEIIRVLQL

    TSWDEDAWAS KDTEAMKRAL ASIDSKLNQA KGWLRDPNAS PGDAGEQAIR

    QILDEAGKVG ELCAGKERRE ILGTCKMLGQ MTDQVADLRA RGQGASPVAM

    QKAQQVSQGL DVLTAKVENA ARKLEAMTNS KQSIAKKIDA AQNWLADPNG

    GPEGEEQIRG ALAEARKIAE LCDDPKERDD ILRSLGEIAA LTSKLGDLRR

    QGKGDSPEAR ALAKQVATAL QNLQTKTNRA VANSRPAKAA VHLEGKIEQA

    QRWIDNPTVD DRGVGQAAIR GLVAEGHRLA NVMMGPYRQD LLAKCDRVDQ

    LTAQLADLAA RGEGESPQAR ALASQLQDSL KDLKAQMQEA MTQEVSDVFS

    DTTTPIKLLA VAATAPPDAP NREEVFDERA ANFENHSGRL GATAEKAAAV

    GTANKSTVEG IQASVKTARE LTPQVISAAR ILLRNPGNQA AYEHFETMKN

    QWIDNVEKMT GLVDEAIDTK SLLDASEEAI KKDLDKCKVA MANIQPQMLV

    AGATSIARRA NRILLVAKRE VENSEDPKFR EAVKAASDEL SKTISPMVMD

    AKAVAGNISD PGLQKSFLDS GYRILGAVAK VREAFQPQEP DFPPPPPDLE

    QLRLTDELAP PKPPLPEGEV PPPRPPPPEE KDEEFPEQKA GEVINQPMMM

    AARQLHDEAR KWSSKGNDII AAAKRMALLM AEMSRLVRGG SGTKRALIQC

    AKDIAKASDE VTRLAKEVAK QCTDKRIRTN LLQVCERIPT ISTQLKILST

    VKATMLGRTN ISDEESEQAT EMLVHNAQNL MQSVKETVRE AEAASIKIRT

    DAGFTLRWVR KTPWYQ

  • UCSC uc007skz.1; mouse.;



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