Human CD209 ELISA Kit

Alternative name
Human C-type lectin domain family 4 member L ELISA Kit;Human dendritic cell-specific ICAM-3-grabbing non-integrin 1 ELISA Kit;Human DC-SIGN ELISA Kit;Human DC-SIGN1 ELISA Kit;Human CLEC4L ELISA Kit;Human CDSIGN ELISA Kit;Human CD209 molecule ELISA Kit;Human CD209 antigen ELISA Kit;Human HIV gpl20-binding protein ELISA Kit;Human dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin ELISA Kit;Human dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin ELISA Kit;
Catalog
E007358
species
Human
GeneCD209
SpecificityThis assay has high sensitivity and excellent specificity for detection of CD209. No significant cross-reactivity or interference between CD209 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD209 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL
Intended UseHuman CD209 ELISA Kit allows for the in vitro quantitative determination of CD209 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalIntended Uses: This CD209 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CD209. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CD209 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD209 antibody and an CD209-HRP conjugate. The assay sample and buffer are incubated together with CD209-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD209 concentration since CD209 from samples and CD209-HRP conjugate compete for the anti-CD209 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD209 from the sample, fewer sites are left to bind CD209-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD209 concentration in each sample is interpolated from this standard curve.
Human CD209 antigen Protein information
Uniprot ID CD209_HUMAN
Uniprot AC Q96QQ8;
UniGene Hs.278694;
GeneID 30835
KEGG hsa:30835;
Human CD209 antigen Protein SEQUENCE
SEQUENCE 404 AA; 45775 MW; A23FA246014533C0 CRC64;
MSDSKEPRLQ QLGLLEEEQL RGLGFRQTRG YKSLAGCLGH GPLVLQLLSF
TLLAGLLVQV SKVPSSISQE QSRQDAIYQN LTQLKAAVGE LSEKSKLQEI
YQELTQLKAA VGELPEKSKL QEIYQELTRL KAAVGELPEK SKLQEIYQEL
TWLKAAVGEL PEKSKMQEIY QELTRLKAAV GELPEKSKQQ EIYQELTRLK
AAVGELPEKS KQQEIYQELT RLKAAVGELP EKSKQQEIYQ ELTQLKAAVE
RLCHPCPWEW TFFQGNCYFM SNSQRNWHDS ITACKEVGAQ LVVIKSAEEQ
NFLQLQSSRS NRFTWMGLSD LNQEGTWQWV DGSPLLPSFK QYWNRGEPNN
VGEEDCAEFS GNGWNDDKCN LAKFWICKKS AASCSRDEEQ FLSPAPATPN
PPPA
UCSC uc002mhr.3; human. [Q9NNX6-1];

Category

Human CD209 ELISA Kit