Human CX3CL1 ELISA Kit

Alternative name
Human C-X3-C motif chemokine 1 ELISA Kit;Human CX3C membrane-anchored chemokine ELISA Kit;Human neurotactin ELISA Kit;Human small-inducible cytokine D1 ELISA Kit;Human FKN ELISA Kit;Human NTT ELISA Kit;Human SCYD1 ELISA Kit;Human ABCD-3 ELISA Kit;Human C3Xkine ELISA Kit;Human CXC3 ELISA Kit;Human CXC3C ELISA Kit;Human NTN ELISA Kit;Human fractalkine ELISA Kit;Human C-X3-C motif chemokine ligand 1 ELISA Kit;Human chemokine (C-X3-C motif) ligand 1 ELISA Kit;Human small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin) ELISA Kit;Human small inducible cytokine subfamily D (Cys-X3-Cys), member-1 ELISA Kit;
Catalog
E008000
species
Human
GeneCX3CL1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CX3CL1. No significant cross-reactivity or interference between Human CX3CL1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity31.25 pg/ml
Intended UseHuman CX3CL1 ELISA Kit allows for the in vitro quantitative determination of CX3CL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fractalkine. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Fractalkine and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Fractalkine, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Fractalkine in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Processed fractalkine Protein information
Uniprot ID X3CL1_HUMAN
Uniprot AC P78423; O00672;
UniGene Hs.531668;
GeneID 6376
KEGG hsa:6376;
Human Processed fractalkine Protein SEQUENCE
SEQUENCE 397 AA; 42203 MW; BAC71F5080DF9CA6 CRC64;
MAPISLSWLL RLATFCHLTV LLAGQHHGVT KCNITCSKMT SKIPVALLIH
YQQNQASCGK RAIILETRQH RLFCADPKEQ WVKDAMQHLD RQAAALTRNG
GTFEKQIGEV KPRTTPAAGG MDESVVLEPE ATGESSSLEP TPSSQEAQRA
LGTSPELPTG VTGSSGTRLP PTPKAQDGGP VGTELFRVPP VSTAATWQSS
APHQPGPSLW AEAKTSEAPS TQDPSTQAST ASSPAPEENA PSEGQRVWGQ
GQSPRPENSL EREEMGPVPA HTDAFQDWGP GSMAHVSVVP VSSEGTPSRE
PVASGSWTPK AEEPIHATMD PQRLGVLITP VPDAQAATRR QAVGLLAFLG
LLFCLGVAMF TYQSLQGCPR KMAGEMAEGL RYIPRSCGSN SYVLVPV
UCSC uc002eli.4; human.;

Category

Human CX3CL1 ELISA Kit