Human CHAD ELISA Kit

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  • Alternative name

    Human cartilage leucine-rich protein ELISA Kit;Human SLRR4A ELISA Kit;Human chondroadherin ELISA Kit;Human chondroadherin proteoglycan ELISA Kit;

  • Catalog
    E008303
  • species
    Human
  • GeneCHAD
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human CHAD. No significant cross-reactivity or interference between human CHAD and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human CHAD is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman CHAD ELISA Kit allows for the in vitro quantitative determination of CHAD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHAD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHAD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHAD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHAD bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Chondroadherin Protein information
  • Uniprot ID CHAD_HUMAN
  • Uniprot AC O15335; A8K812; Q6GTU0; Q96RJ5;
  • UniGene Hs.97220;
  • GeneID 1101
  • KEGG hsa:1101;
  • Human Chondroadherin Protein SEQUENCE
  • SEQUENCE 359 AA; 40476 MW; CF24D2B5A5DFCF0C CRC64;

    MVRPMLLLSL GLLAGLLPAL AACPQNCHCH SDLQHVICDK VGLQKIPKVS

    EKTKLLNLQR NNFPVLAANS FRAMPNLVSL HLQHCQIREV AAGAFRGLKQ

    LIYLYLSHND IRVLRAGAFD DLTELTYLYL DHNKVTELPR GLLSPLVNLF

    ILQLNNNKIR ELRAGAFQGA KDLRWLYLSE NALSSLQPGA LDDVENLAKF

    HVDRNQLSSY PSAALSKLRV VEELKLSHNP LKSIPDNAFQ SFGRYLETLW

    LDNTNLEKFS DGAFLGVTTL KHVHLENNRL NQLPSNFPFD SLETLALTNN

    PWKCTCQLRG LRRWLEAKAS RPDATCASPA KFKGQHIRDT DAFRSCKFPT

    KRSKKAGRH

  • UCSC uc010dbr.4; human.;



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