Human CHADL ELISA Kit

Alternative name
Human SLRR4B ELISA Kit;Human chondroadherin like ELISA Kit;Human chondroadherin-like protein ELISA Kit;
Catalog
E008306
species
Human
GeneCHADL
SpecificityThis assay has high sensitivity and excellent specificity for detection of CHADL. No significant cross-reactivity or interference between CHADL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CHADL and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL
Intended UseHuman CHADL ELISA Kit allows for the in vitro quantitative determination of CHADL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This CHADL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CHADL. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CHADL ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CHADL antibody and an CHADL-HRP conjugate. The assay sample and buffer are incubated together with CHADL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHADL concentration since CHADL from samples and CHADL-HRP conjugate compete for the anti-CHADL antibody binding site. Since the number of sites is limited, as more sites are occupied by CHADL from the sample, fewer sites are left to bind CHADL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHADL concentration in each sample is interpolated from this standard curve.
Human Chondroadherin-like protein Protein information
Uniprot ID CHADL_HUMAN
Uniprot AC Q6NUI6; Q05CY2; Q4G0S0; Q5JY13; Q86XY1; Q96E60;
UniGene Hs.344488;
GeneID 150356
KEGG hsa:150356;
Human Chondroadherin-like protein Protein SEQUENCE
SEQUENCE 762 AA; 82388 MW; BE01DF94D3408A70 CRC64;
MEGPRSSTHV PLVLPLLVLL LLAPARQAAA QRCPQACICD NSRRHVACRY
QNLTEVPDAI PELTQRLDLQ GNLLKVIPAA AFQGVPHLTH LDLRHCEVEL
VAEGAFRGLG RLLLLNLASN HLRELPQEAL DGLGSLRRLE LEGNALEELR
PGTFGALGAL ATLNLAHNAL VYLPAMAFQG LLRVRWLRLS HNALSVLAPE
ALAGLPALRR LSLHHNELQA LPGPVLSQAR GLARLELGHN PLTYAGEEDG
LALPGLRELL LDGGALQALG PRAFAHCPRL HTLDLRGNQL DTLPPLQGPG
QLRRLRLQGN PLWCGCQARP LLEWLARARV RSDGACQGPR RLRGEALDAL
RPWDLRCPGD AAQEEEELEE RAVAGPRAPP RGPPRGPGEE RAVAPCPRAC
VCVPESRHSS CEGCGLQAVP RGFPSDTQLL DLRRNHFPSV PRAAFPGLGH
LVSLHLQHCG IAELEAGALA GLGRLIYLYL SDNQLAGLSA AALEGAPRLG
YLYLERNRFL QVPGAALRAL PSLFSLHLQD NAVDRLAPGD LGRTRALRWV
YLSGNRITEV SLGALGPARE LEKLHLDRNQ LREVPTGALE GLPALLELQL
SGNPLRALRD GAFQPVGRSL QHLFLNSSGL EQICPGAFSG LGPGLQSLHL
QKNQLRALPA LPSLSQLELI DLSSNPFHCD CQLLPLHRWL TGLNLRVGAT
CATPPNARGQ RVKAAAAVFE DCPGWAARKA KRTPASRPSA RRTPIKGRQC
GADKVGKEKG RL
UCSC uc003azq.5; human. [Q6NUI6-1];

Category

Human CHADL ELISA Kit

Human SLRR4B ELISA Kit;Human chondroadherin like ELISA Kit;Human chondroadherin-like protein ELISA Kit;