Human CD300A ELISA Kit

Alternative name
Human CLM-8 ELISA Kit;Human CD300 antigen-like family member A ELISA Kit;Human CMRF-35-H9 ELISA Kit;Human CMRF35-H9 ELISA Kit;Human CMRF35-H ELISA Kit;Human IRC1/IRC2 ELISA Kit;Human immunoglobulin superfamily member 12 ELISA Kit;Human IGSF12 ELISA Kit;Human inhibitory receptor protein 60 ELISA Kit;Human IRp60 ELISA Kit;Human NK inhibitory receptor ELISA Kit;Human CMRF35H ELISA Kit;Human CMRF-35H ELISA Kit;Human CMRF35H9 ELISA Kit;Human IRC1 ELISA Kit;Human IRC2 ELISA Kit;Human CD300a molecule ELISA Kit;Human CMRF35-like molecule 8 ELISA Kit;Human CD300a antigen ELISA Kit;Human CMRF35H leukocyte immunoglobulin-like receptor ELISA Kit;Human leukocyte membrane antigen ELISA Kit;
Catalog
E009770
species
Human
GeneCD300A
SpecificityThis assay has high sensitivity and excellent specificity for detection of CD300A. No significant cross-reactivity or interference between CD300A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD300A and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CD300A ELISA Kit allows for the in vitro quantitative determination of CD300A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalIntended Uses: This CD300A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CD300A. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CD300A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD300A antibody and an CD300A-HRP conjugate. The assay sample and buffer are incubated together with CD300A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD300A concentration since CD300A from samples and CD300A-HRP conjugate compete for the anti-CD300A antibody binding site. Since the number of sites is limited, as more sites are occupied by CD300A from the sample, fewer sites are left to bind CD300A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD300A concentration in each sample is interpolated from this standard curve.
Human CMRF35-like molecule 8 Protein information
Uniprot ID CLM8_HUMAN
Uniprot AC Q9UGN4; A8MW96; O95100; Q9HD97; Q9P0F3; Q9UBK4; Q9UMS9; Q9UMT0;
UniGene Hs.9688;
GeneID 11314
KEGG hsa:11314;
Human CMRF35-like molecule 8 Protein SEQUENCE
SEQUENCE 299 AA; 33201 MW; 978461DA87E86269 CRC64;
MWLPWALLLL WVPGCFALSK CRTVAGPVGG SLSVQCPYEK EHRTLNKYWC
RPPQIFLCDK IVETKGSAGK RNGRVSIRDS PANLSFTVTL ENLTEEDAGT
YWCGVDTPWL RDFHDPVVEV EVSVFPASTS MTPASITAAK TSTITTAFPP
VSSTTLFAVG ATHSASIQEE TEEVVNSQLP LLLSLLALLL LLLVGASLLA
WRMFQKWIKA GDHSELSQNP KQAATQSELH YANLELLMWP LQEKPAPPRE
VEVEYSTVAS PREELHYASV VFDSNTNRIA AQRPREEEPD SDYSVIRKT
UCSC uc002jkv.5; human. [Q9UGN4-1];

Category

Human CD300A ELISA Kit