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Alternative name
ADH8, ADHFE1, FLJ32430, HMFT2263, HOT antibody
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Catalog
A000168
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IP
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PurificationImmunogen affinity purified
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Immunogenalcohol dehydrogenase, iron containing, 1
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IHCImmunohistochemistry of paraffin-embedded human liver tissue slide using A000168( ADHFE1 Antibody) at dilution of 1:50
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Western Blotmouse heart tissue were subjected to SDS PAGE followed by western blot with A000168(ADHFE1 antibody) at dilution of 1:1000
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Recommended dilutionWB :1:1000-1:10000;IHC:1:20-1:200;IP:1:200-1:1000
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Product Description specificalCatalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2- hydroxyglutarate (D-2-HG). D,L-3-hydroxyisobutyrate and L-3- hydroxybutyrate (L-3-OHB) are also substrates for HOT with 10-fold lower activities.
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Gene nameADHFE1
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locationMitochondrion
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Uniprot IDQ8IWW8
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Calculated M.W.50kDa
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Observed M.W.45 kDa
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