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Alternative name
ADP ribosylation factor 1, ARF1 antibody
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Catalog
A000529
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse
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Applications Tested/SuitableELISA,WB,IF,IHC,IP
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PurificationImmunogen affinity purified
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ImmunogenADP-ribosylation factor 1
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IHCImmunohistochemistry of paraffin-embedded human small intestine tissue slide using A000529( ARF1 Antibody) at dilution of 1:50
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Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A000529(ARF1 antibody) at dilution of 1:500
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Recommended dilutionWB : 1:200-1:2000
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Product Description specificalGTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).
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Gene nameARF1
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Uniprot IDP84077
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Observed M.W.21 kDa
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