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Alternative name
FUT12, KIAA0180 antibody
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Catalog
A006602
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IF,IP
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PurificationImmunogen affinity purified
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Immunogenprotein O-fucosyltransferase 1
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IHCImmunohistochemistry of paraffin-embedded human liver cancer using A006602(POFUT1 antibody) at dilution of 1:50
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Western BlotHepG2 cells were subjected to SDS PAGE followed by western blot with A006602(POFUT1 antibody) at dilution of 1:500
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Recommended dilutionWB : 1:200-1:2000
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Product Description specificalCatalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs).
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Gene namePOFUT1
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Uniprot IDQ9H488
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Observed M.W.44kd
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