-
Alternative name
DKFZp586D211, hPot1, POT1 antibody
-
Catalog
A006645
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityPolyclonal Antibody
-
Host Rabbit
-
Species ReactivityHuman, Mouse
-
Applications Tested/SuitableELISA,WB,IHC
-
PurificationImmunogen affinity purified
-
ImmunogenPOT1 protection of telomeres 1 homolog (S. pombe)
-
IHCImmunohistochemistry of paraffin-embedded human prostate cancer using A006645(POT1 antibody) at dilution of 1:100
-
Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A006645(POT1 antibody) at dilution of 1:500
-
Recommended dilutionWB : 1:200-1:2000
-
Product Description specificalComponent of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA- binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'- TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity.
-
Gene namePOT1
-
Uniprot IDQ9NUX5
-
Observed M.W.71 kDa
-