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Alternative name
KIAA0619 antibody
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Catalog
A007378
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IF,IP
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PurificationImmunogen affinity purified
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ImmunogenRho-associated, coiled-coil containing protein kinase 2
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IHCImmunohistochemistry of paraffin-embedded human liver using A007378(ROCK2 antibody) at dilution of 1:100
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Western BlotHT-1080 cells were subjected to SDS PAGE followed by western blot with A007378(ROCK2 antibody) at dilution of 1:1000
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Recommended dilutionWB:1:1000-1:10000
IP:1:200-1:2000
IHC:1:20-1:200
IF:1:10-1:100
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Product Description specificalProtein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
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Uniprot IDO75116
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Calculated M.W.161kDa
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