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Alternative name
DNA annealing helicase and endonuclease ZRANB3,Annealing helicase 2,AH2 antibody
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Catalog
A009756
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman
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Applications Tested/SuitableELISA,WB
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PurificationImmunogen affinity purified
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Immunogenzinc finger, RAN-binding domain containing 3
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Western BlotHEK-293 cells were subjected to SDS PAGE followed by western blot with A009756( ZRANB3 Antibody) at dilution of 1:600
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Recommended dilutionWB:1:200-1:2000;IF:1:20-1:200
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Product Description specificalDNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks.
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Gene nameZRANB3
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locationChromosome,Nucleus
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Uniprot IDQ5FWF4
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Calculated M.W.123kDa,observed 150kDa and 50kDa
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Observed M.W.150 kDa
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