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Catalog
C000723
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Alternative name
Glycogen phosphorylase, muscle form CLIA KIT; MyophosphorylasePYGM CLIA KIT;
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GenePYGM
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Species ReactivityRat
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Detection Range0.156~10ng/mL
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Sensitivity0.094ng/mL
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Product Description specificalThe micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PYGM. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PYGM and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PYGM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PYGM. You can calculate the concentration of Rat PYGM in the samples by comparing the RLU value of the samples to the standard curve.
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