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Human COX6C ELISA Kit

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  • Alternative name

    Human cytochrome c oxidase polypeptide VIc ELISA Kit;Human cytochrome c oxidase subunit 6C ELISA Kit;Human cytochrome c oxidase subunit VIc preprotein ELISA Kit;
  • Catalog
    E010976
  • species
    Human
  • GeneCOX6C
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of COX6C. No significant cross-reactivity or interference between COX6C and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COX6C and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman COX6C ELISA Kit allows for the in vitro quantitative determination of COX6C , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: This COX6C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human COX6C. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||COX6C ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COX6C antibody and an COX6C-HRP conjugate. The assay sample and buffer are incubated together with COX6C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COX6C concentration since COX6C from samples and COX6C-HRP conjugate compete for the anti-COX6C antibody binding site. Since the number of sites is limited, as more sites are occupied by COX6C from the sample, fewer sites are left to bind COX6C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COX6C concentration in each sample is interpolated from this standard curve.
  • Human Cytochrome c oxidase subunit 6C Protein information
  • Uniprot ID COX6C_HUMAN
  • Uniprot AC P09669; B2R4D7;
  • UniGene Hs.351875;
  • GeneID 1345
  • KEGG hsa:1345;
  • Human Cytochrome c oxidase subunit 6C Protein SEQUENCE

  • SEQUENCE 75 AA; 8781 MW; D09FEE0C0AA7A798 CRC64;

    MAPEVLPKPR MRGLLARRLR NHMAVAFVLS LGVAALYKFR VADQRKKAYA

    DFYRNYDVMK DFEEMRKAGI FQSVK

  • UCSC uc003yiy.3; human.;



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