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Human EXT1 ELISA Kit

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  • Alternative name

    Human glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase ELISA Kit;Human multiple exostoses protein 1 ELISA Kit;Human putative tumor suppressor protein EXT1 ELISA Kit;Human EXT ELISA Kit;Human LGCR ELISA Kit;Human LGS ELISA Kit;Human TRPS2 ELISA Kit;Human TTV ELISA Kit;Human exostosin glycosyltransferase 1 ELISA Kit;Human exostosin-1 ELISA Kit;Human Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N- acetylglucosaminyltransferase ELISA Kit;Human Langer-Giedion syndrome chromosome region ELISA Kit;Human N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase ELISA Kit;Human exostoses (multiple) 1 ELISA Kit;
  • Catalog
    E013861
  • species
    Human
  • GeneEXT1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Exostoses 1 (EXT1). No significant cross-reactivity or interference between Exostoses 1 (EXT1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.28ng/mL.
  • Intended UseHuman EXT1 ELISA Kit allows for the in vitro quantitative determination of EXT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Exostoses 1 (EXT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Exostoses 1 (EXT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Exostoses 1 (EXT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Exostoses 1 (EXT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Exostosin-1 Protein information
  • Uniprot ID EXT1_HUMAN
  • Uniprot AC Q16394; B2R7V2; Q9BVI9;
  • UniGene Hs.492618;
  • GeneID 2131
  • KEGG hsa:2131;
  • Human Exostosin-1 Protein SEQUENCE

  • SEQUENCE 746 AA; 86255 MW; 842CD7E6C1312C1A CRC64;

    MQAKKRYFIL LSAGSCLALL FYFGGLQFRA SRSHSRREEH SGRNGLHHPS

    PDHFWPRFPD ALRPFVPWDQ LENEDSSVHI SPRQKRDANS SIYKGKKCRM

    ESCFDFTLCK KNGFKVYVYP QQKGEKIAES YQNILAAIEG SRFYTSDPSQ

    ACLFVLSLDT LDRDQLSPQY VHNLRSKVQS LHLWNNGRNH LIFNLYSGTW

    PDYTEDVGFD IGQAMLAKAS ISTENFRPNF DVSIPLFSKD HPRTGGERGF

    LKFNTIPPLR KYMLVFKGKR YLTGIGSDTR NALYHVHNGE DVVLLTTCKH

    GKDWQKHKDS RCDRDNTEYE KYDYREMLHN ATFCLVPRGR RLGSFRFLEA

    LQAACVPVML SNGWELPFSE VINWNQAAVI GDERLLLQIP STIRSIHQDK

    ILALRQQTQF LWEAYFSSVE KIVLTTLEII QDRIFKHISR NSLIWNKHPG

    GLFVLPQYSS YLGDFPYYYA NLGLKPPSKF TAVIHAVTPL VSQSQPVLKL

    LVAAAKSQYC AQIIVLWNCD KPLPAKHRWP ATAVPVVVIE GESKVMSSRF

    LPYDNIITDA VLSLDEDTVL STTEVDFAFT VWQSFPERIV GYPARSHFWD

    NSKERWGYTS KWTNDYSMVL TGAAIYHKYY HYLYSHYLPA SLKNMVDQLA

    NCEDILMNFL VSAVTKLPPI KVTQKKQYKE TMMGQTSRAS RWADPDHFAQ

    RQSCMNTFAS WFGYMPLIHS QMRLDPVLFK DQVSILRKKY RDIERL

  • UCSC uc003yok.3; human.;



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