Human GOT1 ELISA Kit

Alternative name
Human cAspAT ELISA Kit;Human cysteine aminotransferase, cytoplasmic ELISA Kit;Human cysteine transaminase, cytoplasmic ELISA Kit;Human cCAT ELISA Kit;Human glutamate oxaloacetate transaminase 1 ELISA Kit;Human transaminase A ELISA Kit;Human AST1 ELISA Kit;Human ASTQTL1 ELISA Kit;Human GIG18 ELISA Kit;Human glutamic-oxaloacetic transaminase 1 ELISA Kit;Human aspartate aminotransferase, cytoplasmic ELISA Kit;Human aspartate aminotransferase 1 ELISA Kit;Human aspartate transaminase 1 ELISA Kit;Human glutamic-oxaloacetic transaminase 1, soluble ELISA Kit;Human growth-inhibiting protein 18 ELISA Kit;Human testis secretory sperm-binding protein Li 196a ELISA Kit;
Catalog
E016814
species
Human
GeneGOT1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human GOT1. No significant cross-reactivity or interference between Human GOT1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.78 mIU/ml
Intended UseHuman GOT1 ELISA Kit allows for the in vitro quantitative determination of GOT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: Aspartate transaminase (AST) also called serum glutamic oxaloacetic transaminase (SGOT) or aspartate aminotransferase (ASAT/AAT) is similar to alanine transaminase (ALT) in that it is another enzyme associated with liver parenchymal cells. It is raised in acute liver damage. It is also present in red blood cells and cardiac muscle, skeletal muscle, and kidney and brain tissue, and may be elevated due to damage to those sources as well. AST was defined as a biochemical marker for the diagnosis of acute myocardial infarction in 1954. However the use of AST for such a diagnosis is now redundant and has been superseded by the cardiac troponins. AST (SGOT) is commonly measured clinically as a part of diagnostic liver function tests, to determine liver health. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AST. Standards or samples are then added to the appropriate microtiter plate wells with a 3 biotin-conjugated antibody preparation specific for AST and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain AST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AST in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Aspartate aminotransferase, cytoplasmic Protein information
Uniprot ID AATC_HUMAN
Uniprot AC P17174; B2R6R7; B7Z7E9; Q5VW80;
UniGene Hs.500756;
GeneID 2805
KEGG hsa:2805;
Human Aspartate aminotransferase, cytoplasmic Protein SEQUENCE
SEQUENCE 413 AA; 46248 MW; 69FE68BF0C045219 CRC64;
MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV
LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR
VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA
AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID
PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE
FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP
AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT
WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY
VATSIHEAVT KIQ
UCSC uc001kpr.4; human. [P17174-1];

Category

Human GOT1 ELISA Kit