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Human IL12RB1 ELISA Kit

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  • Alternative name

    Human IL-12 receptor subunit beta-1 ELISA Kit;Human IL-12R subunit beta-1 ELISA Kit;Human IL-12R-beta-1 ELISA Kit;Human IL-12RB1 ELISA Kit;Human IL-12 receptor beta component ELISA Kit;Human IL12R ELISA Kit;Human IL12RB ELISA Kit;Human CD212 ELISA Kit;Human IL-12R-BETA1 ELISA Kit;Human IMD30 ELISA Kit;Human interleukin 12 receptor subunit beta 1 ELISA Kit;Human interleukin-12 receptor subunit beta-1 ELISA Kit;Human cluster of differentiation 212 ELISA Kit;Human interleukin 12 receptor, beta 1 ELISA Kit;Human interleukin-12 receptor beta-1 chain ELISA Kit;
  • Catalog
    E020502
  • species
    Human
  • GeneIL12RB1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 12 Receptor Beta 1 (IL12Rb1). No significant cross-reactivity or interference between Interleukin 12 Receptor Beta 1 (IL12Rb1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman IL12RB1 ELISA Kit allows for the in vitro quantitative determination of IL12RB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12 Receptor Beta 1 (IL12Rb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12 Receptor Beta 1 (IL12Rb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12 Receptor Beta 1 (IL12Rb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 12 Receptor Beta 1 (IL12Rb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Interleukin-12 receptor subunit beta-1 Protein information
  • Uniprot ID I12R1_HUMAN
  • Uniprot AC P42701; A8K308; B2RPF1; B7ZKK3; Q8N6Q7;
  • UniGene Hs.567294;
  • GeneID 3594
  • KEGG hsa:3594;
  • Human Interleukin-12 receptor subunit beta-1 Protein SEQUENCE

  • SEQUENCE 662 AA; 73109 MW; 541ADA60F62DA1EF CRC64;

    MEPLVTWVVP LLFLFLLSRQ GAACRTSECC FQDPPYPDAD SGSASGPRDL

    RCYRISSDRY ECSWQYEGPT AGVSHFLRCC LSSGRCCYFA AGSATRLQFS

    DQAGVSVLYT VTLWVESWAR NQTEKSPEVT LQLYNSVKYE PPLGDIKVSK

    LAGQLRMEWE TPDNQVGAEV QFRHRTPSSP WKLGDCGPQD DDTESCLCPL

    EMNVAQEFQL RRRQLGSQGS SWSKWSSPVC VPPENPPQPQ VRFSVEQLGQ

    DGRRRLTLKE QPTQLELPEG CQGLAPGTEV TYRLQLHMLS CPCKAKATRT

    LHLGKMPYLS GAAYNVAVIS SNQFGPGLNQ TWHIPADTHT EPVALNISVG

    TNGTTMYWPA RAQSMTYCIE WQPVGQDGGL ATCSLTAPQD PDPAGMATYS

    WSRESGAMGQ EKCYYITIFA SAHPEKLTLW STVLSTYHFG GNASAAGTPH

    HVSVKNHSLD SVSVDWAPSL LSTCPGVLKE YVVRCRDEDS KQVSEHPVQP

    TETQVTLSGL RAGVAYTVQV RADTAWLRGV WSQPQRFSIE VQVSDWLIFF

    ASLGSFLSIL LVGVLGYLGL NRAARHLCPP LPTPCASSAI EFPGGKETWQ

    WINPVDFQEE ASLQEALVVE MSWDKGERTE PLEKTELPEG APELALDTEL

    SLEDGDRCKA KM

  • UCSC uc002nhw.2; human. [P42701-1];



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