Human IREB2 ELISA Kit

Alternative name
Human IRE-BP 2 ELISA Kit;Human iron regulatory protein 2 ELISA Kit;Human IRP2 ELISA Kit;Human ACO3 ELISA Kit;Human IRP2AD ELISA Kit;Human iron responsive element binding protein 2 ELISA Kit;Human iron-responsive element-binding protein 2 ELISA Kit;
Catalog
E021064
species
Human
GeneIREB2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Iron Responsive Element Binding Protein 2 (IREB2). No significant cross-reactivity or interference between Iron Responsive Element Binding Protein 2 (IREB2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.059ng/mL.
Intended UseHuman IREB2 ELISA Kit allows for the in vitro quantitative determination of IREB2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Iron Responsive Element Binding Protein 2 (IREB2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Iron Responsive Element Binding Protein 2 (IREB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Iron Responsive Element Binding Protein 2 (IREB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Iron Responsive Element Binding Protein 2 (IREB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Iron-responsive element-binding protein 2 Protein information
Uniprot ID IREB2_HUMAN
Uniprot AC Q9UF17;
UniGene Hs.436031;
GeneID 3658
KEGG hsa:3658;
Human Iron-responsive element-binding protein 2 Protein SEQUENCE
SEQUENCE 963 AA; 105045 MW; F3585B6BEA8DC9CA CRC64;
MDAPKAGYAF EYLIETLNDS SHKKFFDVSK LGTKYDVLPY SIRVLLEAAV
RNCDGFLMKK EDVMNILDWK TKQSNVEVPF FPARVLLQDF TGIPAMVDFA
AMREAVKTLG GDPEKVHPAC PTDLTVDHSL QIDFSKCAIQ NAPNPGGGDL
QKAGKLSPVK VQPKKLPCRG QTTCRGSCDS GELGRNSGTF SSQIENTPIL
CPFHLQPVPE PETVLKNQEV EFGRNRERLQ FFKWSSRVFK NVAVIPPGTG
MAHQINLEYL SRVVFEEKDL LFPDSVVGTD SHITMVNGLG ILGWGVGGIE
TEAVMLGLPV SLTLPEVVGC ELTGSSNPFV TSIDVVLGIT KHLRQVGVAG
KFVEFFGSGV SQLSIVDRTT IANMCPEYGA ILSFFPVDNV TLKHLEHTGF
SKAKLESMET YLKAVKLFRN DQNSSGEPEY SQVIQINLNS IVPSVSGPKR
PQDRVAVTDM KSDFQACLNE KVGFKGFQIA AEKQKDIVSI HYEGSEYKLS
HGSVVIAAVI SCTNNCNPSV MLAAGLLAKK AVEAGLRVKP YIRTSLSPGS
GMVTHYLSSS GVLPYLSKLG FEIVGYGCSI CVGNTAPLSD AVLNAVKQGD
LVTCGILSGN KNFEGRLCDC VRANYLASPP LVVAYAIAGT VNIDFQTEPL
GTDPTGKNIY LHDIWPSREE VHRVEEEHVI LSMFKALKDK IEMGNKRWNS
LEAPDSVLFP WDLKSTYIRC PSFFDKLTKE PIALQAIENA HVLLYLGDSV
TTDHISPAGS IARNSAAAKY LTNRGLTPRE FNSYGARRGN DAVMTRGTFA
NIKLFNKFIG KPAPKTIHFP SGQTLDVFEA AELYQKEGIP LIILAGKKYG
SGNSRDWAAK GPYLLGVKAV LAESYEKIHK DHLIGIGIAP LQFLPGENAD
SLGLSGRETF SLTFPEELSP GITLNIQTST GKVFSVIASF EDDVEITLYK
HGGLLNFVAR KFS
UCSC uc002bdq.4; human. [P48200-1];

Category

Human IREB2 ELISA Kit

Human IRE-BP 2 ELISA Kit;Human iron regulatory protein 2 ELISA Kit;Human IRP2 ELISA Kit;Human ACO3 ELISA Kit;Human IRP2AD ELISA Kit;Human iron responsive element binding protein 2 ELISA Kit;Human iron-responsive element-binding protein 2 ELISA Kit;