Human BIRC7 ELISA Kit

Alternative name
Human kidney inhibitor of apoptosis protein ELISA Kit;Human KIAP ELISA Kit;Human LIVIN ELISA Kit;Human melanoma inhibitor of apoptosis protein ELISA Kit;Human ML-IAP ELISA Kit;Human RING finger protein 50 ELISA Kit;Human RING-type E3 ubiquitin transferase BIRC7 ELISA Kit;Human Truncated livin ELISA Kit;Human p30-Livin ELISA Kit;Human tLivin ELISA Kit;Human MLIAP ELISA Kit;Human RNF50 ELISA Kit;Human baculoviral IAP repeat containing 7 ELISA Kit;Human baculoviral IAP repeat-containing protein 7 ELISA Kit;Human RING-type E3 ubiquitin transferase BIRC7 ELISA Kit;Human livin inhibitor of apoptosis ELISA Kit;
Catalog
E002622
species
Human
GeneBIRC7
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman BIRC7 ELISA Kit allows for the in vitro quantitative determination of BIRC7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the Assay: Anti-livin ELISA kit applies the competitive enzyme immunoassay technique utilizing Anti-livin antigen (Anti-livin) and an Anti-livin-HRP conjugate. The assay sample and buffer are incubated together with Anti-livin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-livin concentration since Anti-livin from samples and Anti-livin-HRP conjugate compete for the Anti-livin antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-livin from the sample, fewer sites are left to bind Anti-livin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-livin concentration in each sample is interpolated from this standard curve
Human Baculoviral IAP repeat-containing protein 7 30kDa subunit Protein information
Uniprot ID BIRC7_HUMAN
Uniprot AC Q96CA5; Q9BQV0; Q9H2A8; Q9HAP7;
UniGene Hs.256126;
GeneID 79444
KEGG hsa:79444;
Human Baculoviral IAP repeat-containing protein 7 30kDa subunit Protein SEQUENCE
SEQUENCE 298 AA; 32798 MW; B2EAAEE531BEC101 CRC64;
MGPKDSAKCL HRGPQPSHWA AGDGPTQERC GPRSLGSPVL GLDTCRAWDH
VDGQILGQLR PLTEEEEEEG AGATLSRGPA FPGMGSEELR LASFYDWPLT
AEVPPELLAA AGFFHTGHQD KVRCFFCYGG LQSWKRGDDP WTEHAKWFPS
CQFLLRSKGR DFVHSVQETH SQLLGSWDPW EEPEDAAPVA PSVPASGYPE
LPTPRREVQS ESAQEPGGVS PAEAQRAWWV LEPPGARDVE AQLRRLQEER
TCKVCLDRAV SIVFVPCGHL VCAECAPGLQ LCPICRAPVR SRVRTFLS
UCSC uc002yei.5; human. [Q96CA5-1];

Category

Human BIRC7 ELISA Kit