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Human WNT9B ELISA Kit

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  • Alternative name

    Human protein Wnt-14b ELISA Kit;Human Protein Wnt-15 ELISA Kit;Human WNT14B ELISA Kit;Human WNT15 ELISA Kit;Human Wnt family member 9B ELISA Kit;Human protein Wnt-9b ELISA Kit;Human wingless-type MMTV integration site family member 9B ELISA Kit;Human wingless-type MMTV integration site family, member 15 ELISA Kit;
  • Catalog
    E031938
  • species
    Human
  • GeneWNT9B
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human WNT9B. No significant cross-reactivity or interference between Human WNT9B and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe limit of detection of Human WNT9B defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 15.6 pg/mL (mean of 6 independent assays).
  • Intended UseHuman WNT9B ELISA Kit allows for the in vitro quantitative determination of WNT9B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitative WNT9B in Human serum, plasma. An antibody specific for WNT9B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT9B present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for WNT9B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT9B bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Protein Wnt-9b Protein information
  • Uniprot ID WNT9B_HUMAN
  • Uniprot AC O14905; Q6UXT4; Q96Q09;
  • UniGene Hs.326420;
  • GeneID 7484
  • KEGG hsa:7484;
  • Human Protein Wnt-9b Protein SEQUENCE

  • SEQUENCE 357 AA; 39001 MW; CC22162F9D294CB8 CRC64;

    MRPPPALALA GLCLLALPAA AASYFGLTGR EVLTPFPGLG TAAAPAQGGA

    HLKQCDLLKL SRRQKQLCRR EPGLAETLRD AAHLGLLECQ FQFRHERWNC

    SLEGRMGLLK RGFKETAFLY AVSSAALTHT LARACSAGRM ERCTCDDSPG

    LESRQAWQWG VCGDNLKYST KFLSNFLGSK RGNKDLRARA DAHNTHVGIK

    AVKSGLRTTC KCHGVSGSCA VRTCWKQLSP FRETGQVLKL RYDSAVKVSS

    ATNEALGRLE LWAPARQGSL TKGLAPRSGD LVYMEDSPSF CRPSKYSPGT

    AGRVCSREAS CSSLCCGRGY DTQSRLVAFS CHCQVQWCCY VECQQCVQEE

    LVYTCKH

  • UCSC uc002ikw.2; human.;



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