Mouse Pld1 ELISA Kit

Alternative name
Mouse PLD 1 ELISA Kit;Mouse mPLD1 ELISA Kit;Mouse Choline phosphatase 1 ELISA Kit;Mouse Phosphatidylcholine-hydrolyzing phospholipase D1 ELISA Kit;
Catalog
E063680
species
Mouse
GenePld1
SpecificityThis assay recognizes recombinant and natural mouse mPLD1. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Pld1 ELISA Kit allows for the in vitro quantitative determination of Pld1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to mPLD1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for mPLD1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain mPLD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of mPLD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Phospholipase D1 Protein information
Uniprot ID PLD1_MOUSE
Uniprot AC Q9Z280; O35911;
UniGene Mm.212039;
Mouse Phospholipase D1 Protein SEQUENCE
SEQUENCE 1074 AA; 123969 MW; E11260982A217280 CRC64;
MSLKSETRVN TSTLQKIAAD MSNLIENLDT RELHFEGEEV EYDASPGDPK
AQEGCIPFSS IYNTQGFKEP NIQTYLSGCP IKAQVLEVER FTSTSRVPSI
NLYTIELTHG EFTWQVKRKF KHFQEFHREL LKYKAFIRIP IPTKRHTFRR
QNVKEEPREM PSLPRSSENA IQEEQFFGRR KQLEDYLTKI LKMPMYRNYH
ATTEFLDVSQ LSFIHDLGPK GLEGMIMKRS GGHRIPGVNC CGHGRACYRW
SKRWLIVKDS FLLYMKPDSG AIAFVLLVDK EFRVKVGRKE TETKYGLRID
NLSRTLILKC NSYRHARWWG GAIEEFIRKH GADFLKDHRF GSYAALHENT
LAKWYVNAKG YFEDIANAME EASEEIFITD WWLSPEIFLK RPVVEGNRWR
LDCILKRKAQ QGVRIFIMLY KEVELALGIN SEYSKRTLMR LHPNIKVMRH
PDHVSSSVYL WAHHEKLVII DQSVAFVGGI DLAYGRWDDN EHRLTDVGSV
KRVTSGLSLG SLTAASVESM ESLSLKDKHE FHKKEPISKI VDETDMKLKG
IGKSRKFSKF SLYRQLHRHH LHNADSISSI DSTSSYFSHC RSHQNLIHGL
KPHLKLFHPS SESEQGLTRH STDTGSIRSV QTGVGELHGE TRFWHGKDYC
NFVFKDWVQL DKPFADFIDR YSTPRMPWHD IGSVVHGKAA RDVARHFIQR
WNFTKIMKPK YRSLSYPFLL PKSQATAHEL RYQVPGAVPA KVQLLRSAAD
WSAGIKHHEE SIHAAYIHVI ENSKHYIYIE NQFFISCADD KVVFNKVGDR
IAQRILKAHR EGQRYRVYIV IPLLPGFEGD ISTGGGNALQ AIMHFNYRTM
CRGESSILEQ LKPELGNKWI NYISFCGLRT HAELEGNLVT ELIYVHSKLL
IADDNTVIIG SANINDRSML GKRDSEMAVI VQDTETVPSV MDGKEYQAGR
FARDLRLECF RLVLGYLSDP SEDLQDPVSD KFFKEIWVST AARNATIYDK
VFRCLPNDEV HNLIQLRDFI NKPILAKEDA LRAEEELRKI RGFLVQFPLY
FLSEENLLPS VGTKEAIVPM EVWT

Category

Mouse Pld1 ELISA Kit

Mouse PLD 1 ELISA Kit;Mouse mPLD1 ELISA Kit;Mouse Choline phosphatase 1 ELISA Kit;Mouse Phosphatidylcholine-hydrolyzing phospholipase D1 ELISA Kit;