How can cultured cells be obtained?
Cell culture
First, the development of antibiotics makes it easy to avoid many of the pollution problems that once plagued cell culture.
The second is the development of technology, such as the use of trypsin to digest cells from the culture bottle, and thus the growing cell lines (such as HeLa cells).
Third, by using these cell lines, scientists can develop standardized, chemically determined cell cultures that are more conducive to cell growth.
The joint development of these three aspects has led more and more scientists to adopt the methods of cell, tissue and organ culture in their research.
In the 1960s and 1970s, the commercialization of this technology further affected cell culture and continued to this day.
Such as corning company began to research and development and sales of disposable plastic and glass cell culture products, improvement of filtration products and materials, liquid and powder tissue culture medium and high throughput drug screening a series of related products.
These and other technological advances have made it more common for cell culture to be applied in a large number of laboratories and industrial enterprises.
The surface of the 150mm plate was staining for the human foreskin.
Cultivate the explants for about two weeks.
Two of the nine explants were not able to grow.
The remaining implants grew well.
Each box is about 2cm wide.
Primitive culture
When surgical methods are used to separate cells from the organism and then place them in an appropriate culture, they can attach, divide and grow.
This is called primary culture.
There are two basic methods of primary culture.
The first is to use the explant to attach the small piece of tissue to the glass or the treated plastic culture bottle and soak it in the medium.
A few days later, individual cells will begin to divide and grow from the tissue explant to the surface or matrix of the culture bottle.
The second approach, is more widely used, the method of mixing digestion in tissue fragments, proteolytic enzymes, such as pancreatic enzyme or collagenase, cells of digestive clouds gathered so as to speed up the steps.
Finally, a single cell suspension was obtained, which was then placed in a cell culture bottle containing culture medium, allowing it to continue to grow and divide.
This method becomes an enzyme solution.
The original culture of bright star flower fish cells was carried out.
embryo
After mincing, the dissociation of pancreatic enzyme was performed.
And then you take the cell
Develop a single layer of cells that fuse in about a week.
Extend the vaccination
When the cells in the original culture bottle have grown and are covered with all the available culture matrix, they must be passed on to provide space for continued growth.
This usually requires the cells to be digested as gently as possible from the substrate by trypsin.
These enzymes are similar to enzymes used in the original culture, which can interrupt the protein keys that make cells adhere to the substrate.
Some cell lines can be collected by gently scraping the cells at the bottom of the vial.
After collection, the cell suspension was further dispersed and placed in a new culture bottle.
When cells too much, you can use the appropriate low temperature protection reagent, such as dimethyl sulfoxide or glycerin to be frozen, and then put in low temperature environment (below - 130 ℃), until you need to use again.