Energy source: glutamine
L-glutamine is an amino acid, an important energy source of cells, involved in protein synthesis and nucleic acid metabolism.
At the same time, l-glutamine is unstable, which can be degraded spontaneously in neutral aqueous solution.
Degradation products are toxic to cells (especially for stem cells).
Can affect cell activity, protein expression level and glycosylation mode.
So how do you solve that?
Scheme two:
choose not to culture medium containing glutamine, add
choice stability of glutamine alternatives
After a steady effort by scientists, a stable alternative to glutamine is finally found: glutaGRO.
Where does it function?
Originally, it was formed by the condensation of l-alanine and l-glutamine, with high stability, which can be preserved at room temperature and stable for 2 years (glutamine needs to be cryopreserved), and it is more convenient to use.
It is not easy to degrade, and does not produce toxic ammonia, which is better for cell growth.
However, it is important to note that this replacement is suitable for mammalian cell culture, but not suitable for insect cell culture system!!!
Energy source: Sodium Pyruvate
Sodium pyruvate can be used as an alternative carbon source in cell culture.
When the glucose is low, cells can metabolize sodium pyruvate to ensure better growth of the cells.
And some cells that have problems with glucose use must be added.
Equilibrium salt solution
The equilibrium salt solution is mainly composed of inorganic salt and glucose.
Its function is to maintain cell osmotic pressure balance, maintain pH stability and provide simple nutrition;
It is also used for the rinsing of tissue blocks, rinsing of cells and other reagents.
It is composed of the friend: PBS Phosphate buffer, DPBS (Dulbecco's Phosphate - Buffered Salines), Earle 's balance salt, Hank's balanced salt.
The pH buffer in the medium
The optimal growth condition for most cells is the pH 6.8 to 7.8.
Most cultures use sodium bicarbonate for buffering, and the buffer of sodium bicarbonate requires carbon dioxide.
The high bicarbonate of sodium (1.5-3.7 g/L) requires 5-10% CO2, and low sodium bicarbonate (0.35g/L) does not require the incubator of carbon dioxide.
Of course, hydrogen ion buffer, such as HEPES, which does not depend on carbon dioxide, has no toxic effect on cells within a certain range.
It can control the constant pH range for a long time to ensure the good growth of the cells.
You can make specific choices based on cell and experiment.
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