What are the likely causes of death or poor cell survival in purchased cells?
1. What are the possible causes of death or poor cell survival in purchased cells?
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The researchers had a poor survival rate during cell culture, and the most common reasons were that the culture medium was poor in using the wrong medium or the culture medium.
Fetal bovine serum use error or fetal bovine serum quality is not good.
Defrost process error.
After freezing, the cells are washed and centrifuged.
Suspended cells are mistaken for dead cells.
Use error to raise temperature.
Cells in a - 80 ℃ for too long.
2. After the frozen tube was thawed, why did the number of cells occur too few?
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In the incubation of frozen cells, the researchers showed a small number of cells, mostly due to errors in the operation of the centrifuge process, causing physical damage to the cells and the loss of cells.
It is recommended that the cells should be defrosted after the cells have been grown overnight and then replaced with the culture medium.
3. What is the function of sodium pyruvate in the medium?
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Pyruvic acid sodium can be used as alternative carbon sources in the cell culture, although the cells tend to be more with glucose as carbon source, however, if there is no glucose, sodium pyruvate can cell metabolism.
4. what medium can be omitted to add phenol red?
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Phenol red is used as an indicator of pH in the medium: it is red in neutral, yellow in acidity and purple in alkalinity.
Studies have shown that phenolic red can mimic the effects of steroid hormones (especially estrogen).
To avoid steroidal reaction, cultured cells, especially mammalian cells, were cultured with non-phenolic red media.
Because of the detection of phenol red interference, some researchers did not use the culture medium with phenol red when doing flow cytometry.
5. Is l-glutamine important in cell culture?
Is it unstable in solution?
Is it unstable in solution?
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L-glutamine is important in cell culture.
After removal of the amino acid, l-glutamine can be used as the energy source of cultured cells to participate in protein synthesis and nucleic acid metabolism.
L-glutamine is degraded after a period of time in a solution, but the exact rate of degradation has not been conclusively determined.
The degradation of l-glutamine leads to the formation of ammonia, which is toxic to some cells.
6. What is the 6glutagro-i (corning 25-015-ci)?
How do cultured cells use glutagro-i?
How stable is this peptide?
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The glutagro-i dipeptide is a derivative of l-glutamine, whose unstable alpha-amino acid is protected by l-alanine.
A peptide enzyme gradually cleaved dipeptide, releasing l-glutamine for utilization.
The glutagro-i dipeptide is very stable, and even in the 20 minutes of 121 pounds of sterilization, the glutagro-i dipeptide solution has the minimum degradation, if under the same conditions, l-glutamine is almost completely degraded.
7. Hank's equilibrium salt solution (HBS) is used in the air and does not require a CO2 incubator.
The reason?
What is the essential difference between Hank's equilibrium salt solution (HBS) and Earle's equilibrium salt solution (EBS)?
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The main difference between HBS and EBS is the level of sodium bicarbonate, which is higher in Eagles (2.2g/L) than in Hanks (0.35 g/L).
The sodium bicarbonate requires a high level of CO2 balance to maintain the pH value of the solution.
The Eagles liquid is in the CO2 at the air level and the solution will be alkaline, and the Hanks liquid will become acidic in the CO2 culture box.
If you want to keep the tissue in the CO2 incubator, you need the Eagles fluid, if it's just the tissue that's going to be stored in the cell culture medium, you can use the Hanks liquid.
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