Sequencing weekly: focusing on three generations of Chinese gene sequencing machines, the latest data is the world's leading!
Recently, the china-us joint papers published on BioRxiv, shows for the first time to use shenzhen house gene GenoCare third generation of single molecule sequencing machine completed the e. coli genome sequencing, with 99.7% accuracy.
It is the third generation of sequencers with the highest accuracy so far.
Shenzhen hanhai genomic biotechnology co., LTD. Is an r&d and manufacturing company of three generations of single-molecule sequencing machines in China, and GenoCare, a single-molecule sequencing system developed by the company, is mainly aimed at clinical applications.
The study was carried out by the southern university of science and technology, rice university of the United States, the Beijing genomics institute and hanhai genomics.
Author will America illumina ii gene sequencing machine and amount GenoCare parallel comparison of the three generations of sequencing machine of e. coli sequencing data, the results show that three generations sequencers GenoCare genome sequencing and illumina instrument the consistency of the results of up to 99%.
The core technology of the GenoCare sequencing machine of hanhai gene is single-molecule fluorescence sequencing.
This technique USES the whole internal reflection imaging method to realize the detection of individual fluorescent molecules without the amplification of PCR and the significant decrease in cost, which has a great advantage in clinical use.
The universal kit of the sequencer was put on record at the end of last year.
Beijing genomics institute, a researcher at the armed force, "said the device not only overcomes the drawback of single molecule sequencing relatively low flux, but also provides the possibility of RNA direct sequencing. Its application will cover the scientific research and clinical two areas, business prospects is infinite."
It is reported that the third generation sequencing machine of the hanhai gene has completed the production of small batch prototype, and will hold the launch of the sequencing instrument on July 31.
It is understood that in 2014, the gene for the ocean was launched in the third generation of single-molecule sequencing systems.
According to China's previous report, on October 27, 2015, the hanhai gene was also published in shenzhen to develop the GenoCare principle prototype of the third-generation single-molecule sequencing machine for clinical use.
At present, independent research and development of general house gene sequencing kit has successfully completed the first kind of medical apparatus and instruments for the record and approval, became the first through the Chinese medical device registration for the record of three generations of single molecule sequencing system kit.
References:
Resequencing the Escherichia coli genome by GenoCare single molecule sequencing platform doi: https://doi.org/10.1101/163089