The 3D genomics articles are published in Nature and Cell, and anno Hi-C is fully analytical

Recently, Chinese scientists published 3D genomics research in Nature and Cell magazine.

Two articles to use the technique of Hi - C to parse the gametes, fertilized ovum and early embryonic cells in mice in 3 d genome structure characteristics, the system reports the mouse chromosome three-dimensional structure in the process of early embryonic development dynamic programming process, for the further study of mammalian early embryos in genome of the three-dimensional structure and fine regulation of important foreshadowing.

Both studies have taken advantage of a Hi - C technology that is very rarely touched, and today we are going to detail Hi - C technology.

• 1.Hi - C definition

Hi - C (High - throughput chromosome conformation capture) technology, it is by 3 C technology development, combined with the selection of biotin labeling and high-throughput sequencing technologies, through crosslinking, steps, such as enzyme digestion, connection within the scope of implementation genome-wide high-throughput detection of chromatin interaction.

Based on the biological information analysis method, the relationship of the entire chromatin DNA in the spatial position of the whole genome was studied, and the three-dimensional structure information of chromatin was obtained.

• 2.Hi - C technology application

Hi - C technology is mainly applied in the direction of genomic assembly, single body map construction and auxiliary macro genome assembly.

Hi - C technology is widely used in tumor research, complex disease research, embryo development and other medical fields.

• 3.The hi-c technical type is fully resolved

At present, hi-c technology is classified according to the content of species and resolution, and it is divided into various technical types, such as hi-c, single-cell hi-c and microorganism hi-c.

In early 2015, general-secretary gene sequencing in the domestic first launched the Hi - C services, including fauna somatic Hi - C - C sequencing and unicellular Hi sequencing service, and will increase the resolution of chromosome three dimensional conformation analysis to 1 KB of the world's top level.

• 4.Hi - C technical indicators

There will be differences in the method of building library of hi-c sequencing technology, and the most important evaluation index of hi-c technology is Valid pair rate.

Solomon's gene is the first domestic offers from Hi - C library building to the data analysis of full service company, is the domestic Hi - C sequencing projects and experience one of the most abundant species gene technology company, general-secretary gene as a three dimensional genomics technology "expert", relying on the international leading technology for building a Hi - C, the analysis process, the strict library quality standards, are in absolute advantage leading 3 d genomics research.

• 5.Anno Hi-C technology major event

In July 2016,

Anno gene collaborated with the French Curie institute and other institutions to analyze the structural characteristics of the lost X chromosome in mice with hi-c technology, and the relevant research results were published in the international top journal Nature

In March 2016,

Anno hi-c implements the new function - assisted genome assembly, elevating genomic assembly levels to chromosome levels

In December 2015.

Solomon's genes and the research achievements of French Curie institute and other units jointly develop HTC - Pro - C (a kind of flexible and efficient standardization Hi analysis process) in Genome Biology, in the same month general-secretary Hi - C further achievements, success will be the Hi - C technology application on the plants such as arabidopsis, rice, Hi - C application extends to the plant kingdom

In November 2012,

Dr. Chan, the chief executive of anno, and Dr. Jobs Dekker, a researcher with the French Curie institute and the inventor of the Hi - C technology, were published in Bioinformatics by the Hi - C analysis software-hitc, developed by professor jodekker

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