The prokaryotic and eukaryotic expression of Asprosin is different

Asprosin, a newly discovered protein hormone, is able to unlock the g-protein - cAMP - PKA pathway, so that glucose in the liver is released quickly into the blood circulation system.
Asprosin goal of treatment is mainly benefited from Ⅱ diabetes and the metabolic syndrome.
So study the structure of Ⅱ diabetes after treatment has a great effect.

The study found that Asprosin was encoded by two exons of the FBN1 gene.
Exon 65 encoding 11 amino acids, exon 66 encoding 129 amino acids, theoretical molecular weight 17kD.
But with anti - Asprosin antibody in serum using western blot method shows only one on sds-page molecular weight of 30 kd protein, but the bacteria expressing recombinant Asprosin 17 kd molecular weight for (A) as shown by mammals Asprosin expressed on sds-page also showed that the molecular weight of 30 kd. So Asprosin is expected to have three N - connection of glycosylation sites and bacteria lack of modification after translation, these can be used to explain mammal and express Asprosin molecular weight different bacteria.

By using the ELISA sandwich method, the standard curve of Asprosin in plasma was obtained by using the recombinant Asprosin, so Asprosin's ELISA sandwich method was of high specificity.

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