With the development of cell culture, the quality of the medium is critical, and the animal serum in the composition of the medium plays an important role in the growth and reproduction of cells.
In the animal serum bovine serum application is the most widely, so the quality of the bovine serum is good or not directly affect the quality and safety of biological products, ensure the quality of bovine serum is also promoting the important link of the biological products quality improvement.
If you buy serum, you must know the following.
Serum function
It provides growth hormone, nutrients, and low molecular nutrients
Provide binding protein
Can combine or adjust the material vitality they combine
It is the source of cell wall and growth factor
The acid alkalinity buffer is used
To provide a protease inhibitor
When the cell is passed on, the remaining trypsin is inactivated and the cells are protected from injury
It can be combined with toxic metals and heat sources to detoxify
Serum levels of classification
Fetal bovine serum: an eight-month old cow heart piercing blood
Newborn bovine serum: neonatal bovine vein extraction from 12 to 24 hours
Calf serum: the birth of the third month old calf artery blood separation serum
Serum requirements
Serum source
From domestic non-epidemic areas, there are documentary evidence on the source of serum, including all information, test results and delivery of the collection process.
Serum collected
Fetal bovine serum is the blood of the heart puncture, newborn cattle (10 ~ 14 days) and calves (10 months).
The serum was collected at low temperature, separated and separated immediately after separation, which met the standard of industrial production.
Serum preparation
1. Thermal inactivated serum (non-essential)
2.Gamma ray irradiation: gamma ray irradiation can effectively inactivate the virus and mycoplasma that may be contaminated in the serum.
3. Sterilizing: the fetal bovine serum should be filtered through three layers of 100nm.
Serum quality inspection
Essay, chemical test
A biochemical test: cholesterol, triglyceride, bilirubin, urea, uric acid, glucose, glutamate transaminase, high tube, progesterone, estradiol and other tests
B trace element detection: potassium, sodium, iron, chloride, calcium, magnesium, total iron binding force, serum sialic acid and other tests
C protein detection: albumin and globulin detection
Microbiological examination
Bacteria and fungi: direct culture
2. Mycoplasma detection: culture method, DNA fluorescence staining
Virus detection: direct fluorescent antibody detection
Bovin blue tongue;
Bovine Adenovirus Bovine Adenovirus;
Bovine Parvovirus; Bovine Parvovirus;
Bovine Viral diarrhea hea Virus;
Rabies Rabies of Rabies;
Reovirus of the exenteral arc virus;
Cattle respiratory syncytial virus BRSV;
Deputy Ⅲ type Parainfluenza virus.
The above is the serum quality inspection, if you purchase, please check whether the above indicators are qualified, so as not to purchase illegal channel serum and influence your research progress.
Buy the serum please identify the regular source and the formal qualification brand, find the agent of the brand serum.
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