We're always saying that before we use fetal bovine serum, we're going to have a thermal inactivation, and that's going to require thermal inactivation before we're going to have any cells or any kind of experiment.
The purpose of thermal inactivation is to remove the heat-sensitive substances in serum, but the inactivation of complement in fetal bovine serum is not necessary.
Researchers have to complement components of commercial fetal bovine serum were determined, they found that fetal bovine serum containing C1, C6 animal serum 1-3%, only a year and other complement components is only 5-50% of adult animals, as for the main components of the complement C3 in fetal bovine serum is almost not checked out.
Through the rehydration fixation experiment, we also obtained similar results in multiple batches of fetal bovine serum.
Fetal bovine serum, therefore, in most of the cell culture is not to need to inactivated, but in the study of immunology, cultivation of ES cells, insect cells and smooth muscle cells, it is recommended to use heat inactivated serum.
Under normal operation, thermal inactivation often has negative effects on serum products and reduces the ability to support cell growth.
In addition, the heating of the serum often results in the precipitation in the serum, and the resulting precipitation is often thought to be the contamination of microorganisms, thus causing unnecessary trouble to users.
Conclusion: only recommended in immunology heat inactivated oh, because heat inactivated can inactivate the complement system appears, the activation of complement in complement cells, stimulate the smooth muscle contraction, the nucleus platelets release of histamine, activation of lymphocytes and macrophages.
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