How to efficiently find plasmid maps

Do molecular experiments, often dealing with different plasmids, to understand the various plastid map information is necessary, today to give you about how to find the plasmid map.

1、Use Vector NT software

nvitrogen's software is definitely the gospel of molecular biology insects. To understand the plasmid map more intuitive, install the software is very necessary.

The software package contains all of the plasmid map information and other more common and classic plasmid maps from Invitrogen.

2、Find the site for the plasmid map

▲Vector Database (addgene)

This site is very user-friendly page, formerly known as lablife, and now the site has been integrated, such as looking for pRS plasmid map, directly in the search box input pRS, you can see a total of more than 30 such a plasmid.

Find the name of the plasmid you need, click to enter, you can see the plasmid map.

Take the first plasmid pRSII413 for example, as shown above, plasmid map is not ugly? Yes, I also feel ugly, it does not matter, see view sequence? Click to enter, we get the sequence of the plasmid map.

▲Vector DB

This page is relatively simple, but the classification is more intuitive, summary and classification are relatively complete. Which basically includes plasmid information for all expression systems.

But the only thing that is not uncomfortable is that this site does not search the bar, too humane. So how do you find the information of the plasmid we need in so much plasmid information? It does not matter, we have a page character search function, see the red mark part.

Method is: web page toolbar editor --- find on this page, and then enter your plasmid name, you can. For example: we enter pRS, is not the same as the search bar and useful information?

Click on the plasmid we need to enter the page to the plasmid pRS303, as shown in the following figure: useful information in addition to the plasmid for some descriptive language, the most important thing to be the red mark part, Sequence Link into the plasmid sequence. NCBI Link Click to enter NCBI directly, how to get the plasmid map in NCBI, the following will be explained in detail.

▲NCBI

Focus on how to use NCBI to find plasmid map information. As shown below, Search drop-down box, select Nucleotide, the search field enter the plasmid name, take pRS303 Example:

Search, the search results, as shown below, click on the right of the send to, select file, genebank and other options, click the Create File option to get a gb format file, import vector software, we need to get the plasmid map.

▲Google and Google Academic

Google is really a good thing, students should learn a good learning under its use, although the total time for the wall can not be opened, but the search efficiency is really high.

Search the formula: plasmid name + map

Plasmid name + sequence + filetype: txt or filetype: pdf

Or: Go to google and click on "Image Search" to find the map directly.

▲Some other nucleic acid databases

European molecular biology experiment

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