Find the method of plasmid mapping

There are two ways to find a plasmid map: Web site using Vector NT software and finding plasmid maps.

Now teach you three other ways.

3、Some important reagent company web pages

In fact, some sites, because these sites in addition to get the plasmid map and other information, you can get on the use of plasmid information, so alone out to do a classification. Now a lot of plasmids, especially the application of a wide range of plasmids are some of the company's commercialized plasmids, basically by a plasmid you will think of a company's name.

Such as simple, clonal vectors pMD18-T, TaKaRa; pGM-T, root; some expression vectors, using the most extensive, pET series, Novagen (Merck); dual MCS Duet series vectors, Novagen; Pichia pastoris expression plasmids pPIC3.5k, pPIC9k, pPICZA, pPICZaA and so on, are Invitrogen; others pYES Saccharomyces cerevisiae expression system, also Invitrogen, so know which common plasmid is to go to their company website You can find information about the plasmid.

In their company's home page search bar directly search for the name of the plasmid, get the plasmid sequence, what is not a problem, and can be down to the expression system operating manual, prokaryotic expression pET expression system operating manual, eukaryotic system read the red yeast expression system The.

4、How to find the plasmid map of the modified plasmid

Some of the plasmid is modified, so the above method can not query the corresponding information. At this point, you can enter the name of the plasmid in google scholar, you can visually see which scholars in the article used in the plasmid, which can understand the source of the plasmid or to the author to consult or request.

Google scholar: http://scholar.google.com/

In addition, we describe how to find out the map of the transformed plasmid by literature, Kuhlman, T. E. and E. C. Cox (2010). "Site-specific chromosomal integration of largeitable constructs." Nucleic Acids Res 38 (6): e92.

This paper presents a method for the integration of chromosomes in Escherichia coli. The three plasmids are described by the author. How can we find these three plasmid maps?

First in PubMed search for the document, as shown below.

Many people find the literature, just download the literature on the OK, in fact, there are many useful information, such as the article's supplementary, as well as the right part of the mark on the right part, Nucleotide, protein is some very important information. Click on the right side of the Nucleotide, enter the following page, the text involved in the six plasmid sequences are all, click to enter, according to the method two, NCBI download plasmid map method can get these plasmids of the map, of course, the premise is the article The author has uploaded the carrier information to NCBI.

5、Forum for help

This should be considered a new way to get a new way to get a good map of placenta, go to some of the more well-known forum.

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